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Etablering af en High-throughput Opsætning til screening små molekyler, der modulerer c-di-GMP Signalering i<em> Pseudomonas aeruginosa</em
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Etablering af en High-throughput Opsætning til screening små molekyler, der modulerer c-di-GMP Signalering i<em> Pseudomonas aeruginosa</em

DOI:
10.3791/54115-v

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11:31 min

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June 30, 2016

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, ,
1Division of Molecular Microbiology, College of Life Sciences,University of Dundee

Capítulos

  • 00:05Título
  • 00:59Generation of GFP Tagged c-di-GMP Reporter P. aeruginosa Strain
  • 04:21Preparation of the Starter Culture for Inoculation
  • 05:35Inoculation and Incubation of the 384-well Plates Containing Small Molecules
  • 06:33Measurement and Analysis of Growth and Intracellular c-di-GMP Level
  • 08:25Results: Hits Obtained Following the High-throughput Screen against P. aeruginosa
  • 10:07Conclusion

Summary

Tadução automática

Tadução automática

Denne artikel beskriver high-throughput assay, som er blevet etableret for at screene store biblioteker af små molekyler for deres potentielle evne til at manipulere cellulære niveauer af cyclisk di-GMP i Pseudomonas aeruginosa, hvilket giver en ny kraftfuldt værktøj til antibakteriel lægemiddelforskning og forbindelse testning.

Tags

High-throughputScreeningSmall MoleculesModulateC-di-GMPPseudomonas AeruginosaSecond MessengerBio-reporterCyclic-di-GMPNovel TherapiesImmune SystemAntibioticsElectroporationCdrA PromoterGreen Fluorescent Protein

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