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Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

JoVE Journal
Medicina

This content is Free Access.

JoVE Journal Medicina

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

DOI:

10.3791/54117-v

•

09:36 min

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July 02, 2016

•

Christian B. Matranga, Adrianne Gladden-Young, James Qu, Sarah Winnicki, Dolo Nosamiefan, Joshua Z. Levin, Pardis C. Sabeti²

¹Broad Institute of MIT and Harvard, ²Harvard University

Capítulos

00:05Título
01:11Selective Depletion of Ribosomal and Carrier RNA from Viral RNA Sample
03:48cDNA Synthesis
05:41DNA Library Construction
07:32Results: RNA Virus Sequencing Results
08:17Conclusion

Summary

Tadução automática

This protocol describes a rapid and broadly applicable method for unbiased RNA-sequencing of viral samples from human clinical isolates.

Tags

Keywords: RNA Viruses Viral Genomics Viral Evolution Unbiased Sequencing Lassa Fever CDNA Synthesis DNA Library Construction Ribosomal RNA Depletion RNase H DNase Treatment

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