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表現型および慢性リンパ球性脈絡髄膜炎ウイルスに感染したマウスから単離された活性化制御性T細胞の機能解析
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
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表現型および慢性リンパ球性脈絡髄膜炎ウイルスに感染したマウスから単離された活性化制御性T細胞の機能解析

DOI:
10.3791/54138-v

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07:17 min

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June 22, 2016

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, ,
1Department of Biochemistry, College of Life Science & Biotechnology,Yonsei University

Capítulos

  • 00:05Título
  • 00:48Conventional (Tconv) and Regulatory (Treg) Splenic T Cell Phenotyping
  • 01:58In Vitro Suppression Assay Using CD4+CD25+ Treg and CD8+ T Cells
  • 03:16CD8+ T Cytokine Production and Cell Proliferation Analyses
  • 04:26Results: Activated Treg Cell-mediated Suppression of the CD8+ Responder T Cell Response
  • 06:16Conclusion

Summary

Tadução automática

Tadução automática

Here, we describe a protocol to analyze the phenotype of regulatory T (Treg) cells isolated from naïve and chronic lymphocytic choriomeningitis virus-infected mice. In addition, we provide a process to evaluate the suppressive activity of the Treg cells.

Tags

Keywords: Regulatory T CellsChronic Lymphocytic Choriomeningitis VirusPhenotypic AnalysisFunctional AnalysisIn Vitro SuppressionCD4+CD25+ CellsCD8+ Responder T CellsAnti-CD3/CD28 BeadsCytokine Production

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