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Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
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Biologia
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JoVE Journal Biologia
Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

DOI:
10.3791/54141-v

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08:51 min

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June 23, 2016

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,
1Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology,Sogang University

Capítulos

  • 00:05Título
  • 00:40Cleaning of the Coverslips
  • 01:54Aminosilanization of the Coverslips
  • 03:01PEGylation of the Coverslips
  • 04:44Assembly of the Flow Chamber
  • 06:15Loading Samples into the Flow Chamber
  • 07:40Results: Stretched DNA Molecules under Flow
  • 08:22Conclusion

Summary

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Tadução automática

We present an approach for visualizing fluorescent protein DNA binding peptide (FP-DBP)-stained large DNA molecules tethered on the polyethylene glycol (PEG) and avidin-coated glass surface and stretched with microfluidic shear flows.

Tags

Keywords: DNA VisualizationSingle Molecule DNADNA Binding ProteinsFluorescent Protein DNA Binding PeptidePiranha EtchingAminosilanizationBiotin PEG Succinimidyl CarbonatePEG Succinimidyl ValerateEpifluorescent MicroscopeDNA Analysis System

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