/

Imunologia e Infecção

/

Detecting Cortex Fragments During Bacterial Spore Germination

JoVE Journal
Imunologia e Infecção

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Imunologia e Infecção

Detecting Cortex Fragments During Bacterial Spore Germination

Detecting Cortex Fragments During Bacterial Spore Germination

DOI:

10.3791/54146-v

•

08:35 min

•

June 25, 2016

•

Michael B. Francis, Joseph A. Sorg

¹Department of Biology,Texas A&M University

Capítulos

00:05Título
00:34Generating Samples
03:20Measuring Cortex Fragments
05:02Addition of Color Reagent
06:46Results: Representative Germination Assay
08:03Conclusion

Summary

Tadução automática

Herein, we describe a colorimetric assay to detect the presence of reducing sugars during bacterial spore germination.

Tags

Keywords: Bacterial Spore Germination Cortex Fragments Reducing Sugars Clostridium Difficile Germination Solution OD600 Centrifugation N-acetylglucosamine Dipicolinic Acid

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

Bioluminescent Bacterial Imaging In Vivo

Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

Electroporation of Functional Bacterial Effectors into Mammalian Cells

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa

Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens

A High-throughput Shigella-specific Bactericidal Assay

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Read Article