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Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
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JoVE Journal Genética
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

DOI:
10.3791/54165-v

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09:43 min

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October 30, 2016

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, , , , ,
1Institut für Medizinische Mikrobiologie,Universitätsmedizin Göttingen

Capítulos

  • 00:05Título
  • 01:11Preparation of Matrix Solution and MALDI-TOF Mass Spectrometry
  • 04:24Verify the Internal Calibration Procedure
  • 04:53Identify Biomarker Ions in the Reference Strain
  • 06:13Assess Biomarker Variability in the Population
  • 06:42Results: MSPP and MLST Analysis of Campylobacter jejuni ssp. doylei Isolates
  • 08:24Conclusion

Summary

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Mass spectrometry-based phyloproteomics (MSPP) was used to type a collection of Campylobacter jejuni ssp. doylei isolates at the strain level in comparison to multilocus sequence typing (MLST).

Tags

Keywords: Campylobacter Jejuni Ssp. DoyleiMass SpectrometryPhyloproteomicsMSPPSubtypingBacteriaEpidemiologyHygieneNosocomial InfectionsDrug ResistanceVirulenceHCCAMatrix SolutionMALDIInternal CalibrateRecombinant Human InsulinBacterial ColoniesFormic AcidAcetonitrile

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