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Methods for Acute and Subacute Murine Hindlimb Ischemia
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JoVE Journal Medicina
Methods for Acute and Subacute Murine Hindlimb Ischemia

Methods for Acute and Subacute Murine Hindlimb Ischemia

DOI:
10.3791/54166-v

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07:57 min

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June 21, 2016

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, , ,
1Division of Cardiology, Department of Medicine,Duke University Medical Center

Capítulos

  • 00:05Título
  • 00:48Limb Ischemia Induction
  • 05:55Results: Perfusion Imaging Variations after Hindlimb Ischemia Induction
  • 06:54Conclusion

Summary

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Surgical induction of hindlimb ischemia in the mouse is useful to examine angiogenesis, however this is compromised in certain inbred mouse strains that display marked ischemia-induced tissue necrosis. Methods are described to induce subacute limb ischemia using ameroid constrictors to circumvent this problem through the induction of gradual arterial occlusion.

Tags

Acute Hindlimb IschemiaSubacute Hindlimb IschemiaPeripheral Artery DiseaseFemoral ArterySuture LigationAmeroid ConstrictorMouse ModelSurgical Procedure

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