É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 00:38Production of X. laevis Embryo Extract
- 07:13Nuclear Shrinking Assay and Immunofluorescence
- 11:28Results: Nuclear Size Regulation Using Xenopus laevis Embryo Extracts
- 13:12Conclusion
Mechanisms of cellular and intra-cellular scaling remain elusive. The use of Xenopus embryo extracts has become increasingly common to elucidate mechanisms of organelle size regulation. This method describes embryo extract preparation and a novel nuclear scaling assay through which mechanisms of nuclear size regulation can be identified.
