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Immunology and Infection

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JoVE Journal Immunology and Infection
Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
 

Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice

Article DOI: 10.3791/54196-v • 09:08 min • July 11th, 2016
July 11th, 2016 •
1, 1, 1, 1, 1
1Department of Pediatrics, Baylor College of Medicine

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We present a rapid and flexible protocol for a single T cell receptor (TCR) retroviral-based in vivo expression system. Retroviral vectors are used to transduce bone marrow progenitor cells to study T cell development and function of a single TCR in vivo as an alternative to TCR transgenic mice.

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Keywords: Retroviral Transduction Bone Marrow Progenitor Cells T-cell Receptor Retrogenic Mice Immunology TCR Bone Marrow Extraction Cell Isolation Red Blood Cell Lysis
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