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在剪切粘结力的测定T淋巴细胞粘附分子相互作用的研究

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

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在剪切粘结力的测定T淋巴细胞粘附分子相互作用的研究

DOI:

10.3791/54203-v

•

07:40 min

•

June 29, 2016

•

Marianne Strazza, Inbar Azoulay-Alfaguter, Michael Peled, Adam Mor²

¹Department of Medicine,New York University School of Medicine, ²Department of Pathology,New York University School of Medicine

Capítulos

00:05Título
00:48Chinese Hamster Ovary (CHO)-Intercellular Adhesion Molecule (ICAM) Cell Plating
01:50T Cell Preparation
02:46Input/Output Hosing and Syringe Pump Preparation
03:59Flow Chamber Loading and Cell Stimulation
05:53Results: SDF-1alpha Induced T Cell Adhesion to ICAM-1 Under Shear Stress
06:51Conclusion

Summary

Tadução automática

这种流动粘附实验提供了T细胞的上皮细胞间的相互作用一个简单的，高冲击的模型。的注射器泵用于产生剪切应力，和共聚焦显微镜捕捉图像进行定量。这些研究的目标是使用流动条件，有效地量化的T细胞粘附。

Tags

Adhesion Shear Stress T Lymphocyte Adhesion Molecule Integrin LFA1 CHO-ICAM T Cells CFSE Flow Chamber Live Imaging

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