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En nem metode til Plant Polysomprofiler profilering
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Biologia
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JoVE Journal Biologia
An Easy Method for Plant Polysome Profiling

En nem metode til Plant Polysomprofiler profilering

DOI:
10.3791/54231-v

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11:09 min

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August 28, 2016

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, , , ,
1Laboratoire de Génétique et Biophysique des Plantes,Aix-Marseille Université, 2UMR 7265 Biologie Végétale & Microbiologie Environnementales,CNRS, 3BIAM,CEA, 4Department of Biology, Biocenter,University of Copenhagen, 5Laboratoire de Chimie Bactérienne, 6CNRS, LCB UMR 7283,Aix Marseille Université

Capítulos

  • 00:00Título
  • 01:34Preparation of Sucrose Gradient
  • 02:51Preparation of Cytosolic Extracts
  • 04:09Polysome Profiling
  • 07:07RNA Extraction
  • 09:17Resultados
  • 10:25Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes an easy method to extract and fractionate transcripts from plant tissues on the basis of the number of bound ribosomes. It allows a global estimate of translation activity and the determination of the translational status of specific mRNAs.

Tags

Keywords: Plant Polysome ProfilingPolysome IsolationMonosome IsolationRNA ExtractionSucrose GradientArabidopsis ThalianaSeedlingUV SpectrophotometryTranslation Regulation

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