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Isolering af Perivaskulær multipotente precursorcelle populationer fra humant hjertevæv
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1Centre for Cardiovascular Science, Queen’s Medical Research Institute,University of Edinburgh, 2Department of Bioengineering and Orthopaedic Surgery,University of Pittsburgh, 3Research Laboratory of Electronics and Department of Biological Engineering,Massachusetts Institute of Technology, 4MRC Centre for Regenerative Medicine,University of Edinburgh, 5Stem Cell Research Center, Department of Orthopaedic Surgery,University of Pittsburgh, 6Department of Orthopaedic Surgery,University of Texas Health Science Center at Houston, 7Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine,University of California at Los Angeles
Capítulos
- 00:05Título
- 01:06Tissue Digestion and Cell Isolation
- 03:27Cell Labelling and Sorting
- 06:05Cell Culture
- 06:54Results: FAS Purification of Myocardial Perivascular Cells and Perivascular Cell Morphology
- 07:39Conclusion
Humant hjertevæv huser multipotente perivaskulære precursor cellepopulationer, som kan være egnede til myocardial regenerering. Den her beskrevne teknik muliggør den samtidige isolering og oprensning af to multipotente stromale cellepopulationer forbundet med native blodkar, dvs. CD146 + CD34 - pericytter og CD34 + CD146 - adventitiaceller celler, fra den humane myocardium.
