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Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
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JoVE Journal Bioengenharia
Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

DOI:
10.3791/54258-v

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08:23 min

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July 10, 2016

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, , , ,
1Department of Biological Engineering,Inha University, 2Department of Mechanical Engineering,Inha University, 3Biohybrid Systems Research Center (BSRC),Inha University, 4Convergent Research Center for Metabolism and Immunoregulation (CRCMI),Inha University

Capítulos

  • 00:05Título
  • 00:32Lipid Bilayer Solutions Preparation
  • 01:38Formation of a Polydimethylsiloxane (PDMS) Thin Film
  • 02:45Chamber Fabrication and Assembly
  • 03:36Membrane Formation and Verification
  • 04:47Electrical Recording of the Lipid Bilayer
  • 05:42Gramicidin A Ion Channel Incorporation
  • 06:58Results: Measuring the Incorporation of Gramicidin A into a Lipid Bilayer
  • 07:46Conclusion

Summary

Tadução automática

Tadução automática

We demonstrate a storable, transportable lipid bilayer formation system. A lipid bilayer membrane can be formed within 1 hr with over 80% success rate when a frozen membrane precursor is brought to ambient temperature. This system will reduce laborious processes and expertise associated with ion channels.

Tags

Keywords: Lipid BilayerPolydimethylsiloxane (PDMS)Thin FilmAutomated Membrane FormationNanobiosensorMembrane-protein InteractionsBuffer SolutionLipid SolutionPre-paintingBlack Lipid Membrane ChamberPTFECNC Machining

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