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自动化脂双层膜的形成使用聚二甲基硅氧烷薄膜

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

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自动化脂双层膜的形成使用聚二甲基硅氧烷薄膜

DOI:

10.3791/54258-v

•

08:23 min

•

July 10, 2016

•

Sangbaek Choi³, Sunhee Yoon³, Hyunil Ryu³, Sun Min Kim^3,4, Tae-Joon Jeon^3,4

¹Department of Biological Engineering,Inha University, ²Department of Mechanical Engineering,Inha University, ³Biohybrid Systems Research Center (BSRC),Inha University, ⁴Convergent Research Center for Metabolism and Immunoregulation (CRCMI),Inha University

Capítulos

00:05Título
00:32Lipid Bilayer Solutions Preparation
01:38Formation of a Polydimethylsiloxane (PDMS) Thin Film
02:45Chamber Fabrication and Assembly
03:36Membrane Formation and Verification
04:47Electrical Recording of the Lipid Bilayer
05:42Gramicidin A Ion Channel Incorporation
06:58Results: Measuring the Incorporation of Gramicidin A into a Lipid Bilayer
07:46Conclusion

Summary

Tadução automática

我们展示了一个可存储，运输的脂质双分子层形成系统。的脂质双层膜可以在1小时内超过80％的成功率，当冷冻膜前体达到环境温度下形成。该系统将减少与离子通道相关的繁琐流程和专业知识。

Tags

Lipid Bilayer Polydimethylsiloxane (PDMS)Thin Film Automated Membrane Formation Nanobiosensor Membrane-protein Interactions Buffer Solution Lipid Solution Pre-painting Black Lipid Membrane Chamber PTFE CNC Machining

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