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उच्च संकल्प समय चूक इमेजिंग और जीवित मानव नाल की शिरा endothelial कोशिकाओं में microtubule गतिशीलता की स्वचालित विश्लेषण
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
DOI:

10:25 min

August 13, 2016

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Capítulos

  • 00:05Título
  • 01:03Coverslip Preparation Prior to Human Umbilical Vein Endothelial Cell (HUVEC) Culture
  • 02:06cDNA Transfection and HUVEC Culture on Coverslip
  • 03:36Preparation of Specimen for Imaging
  • 05:14Cell Migration Assay
  • 07:24Representative Imaging Results
  • 09:41Conclusion

Summary

Tadução automática

Protocols for Human Umbilical Vein Endothelial Cell (HUVEC) culture, transient transfection of fluorescently-labeled markers of microtubule growth, live-cell imaging and automated analysis of interphase microtubule growth dynamics are detailed.

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