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JoVE Journal Developmental Biology
Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
 

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

Article DOI: 10.3791/54276-v • 12:21 min • July 25th, 2016
July 25th, 2016 •
*1,2,3,8, *1,8, 1, 6,7, 1, 6,7, 2,3,4, *2,3, *1,5
1Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, 2Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, 3St. Vincent´s Clinical School, Faculty of Medicine, University of New South Wales, 4School of Biotechnology and Biomolecular Sciences, University of New South Wales, 5Department of Developmental Biology, University of Science and Culture, 6Heart Centre for Children, The Children´s Hospital at Westmead, 7Sydney Medical School, University of Sydney, 8Department of Developmental Biology, University of Science and Culture, Tehran, Iran
* These authors contributed equally

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Resumo
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Here, we present a robust, fast and scalable cardiomyocyte differentiation protocol for human pluripotent stem cells (hPSCs). Cardiomyocytes derived using this large-scale method can provide sufficient cell numbers for their effective use in human cardiovascular disease modeling, high-throughput drug screening, and potentially clinical applications.

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Keyword Extraction: Cardiomyocytes Human Pluripotent Stem Cells Differentiation Protocol High Efficiency Reproducibility Cardiac Disease Modeling Human Induced Pluripotent Stem Cells Cost-effective Human Embryonic Stem Cells Single Cell Passaging Spheroids PBS Trypsin EDTA
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