/
/
Production à grande échelle de cardiomyocytes à partir pluripotentes humaines cellules souches en utilisant un petit protocole Différenciation Molecule-Based hautement reproductible
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Department of Stem Cells and Developmental Biology, Cell Science Research Center,Royan Institute for Stem Cell Biology and Technology, ACECR, 2Developmental and Stem Cell Biology Division,Victor Chang Cardiac Research Institute, 3St. Vincent´s Clinical School, Faculty of Medicine,University of New South Wales, 4School of Biotechnology and Biomolecular Sciences,University of New South Wales, 5Department of Developmental Biology,University of Science and Culture, 6Heart Centre for Children,The Children´s Hospital at Westmead, 7Sydney Medical School,University of Sydney, 8Department of Developmental Biology,University of Science and Culture, Tehran, Iran
Capítulos
- 00:05Título
- 01:03Spinner Flask Internal Surface Silicon Coating
- 02:42hPSC Spheroid Stirred Suspension Bioreactor Differentiation
- 06:42hPSC Differentiation Using Cultures Not Adapted to Single Cell Passaging
- 09:46Results: Representative Cardiomyocyte Differentiation From Various hPSCs Cultures
- 11:19Conclusion
Here, we present a robust, fast and scalable cardiomyocyte differentiation protocol for human pluripotent stem cells (hPSCs). Cardiomyocytes derived using this large-scale method can provide sufficient cell numbers for their effective use in human cardiovascular disease modeling, high-throughput drug screening, and potentially clinical applications.
