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فحص الإنتاجية العالية من الكربوهيدرات المهينة الانزيمات عن طريق رواية غير قابل للذوبان اللونية الركيزة الفحص أطقم
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
DOI:

06:51 min

September 20, 2016

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Capítulos

  • 00:05Título
  • 00:40Chromogenic Assay with CPH Substrates, Detection, and Quantification
  • 02:41Chromogenic Assay with Insoluble Chromogenic Biomass Substrates, Detection, and Quantification
  • 04:15Results: Enzymatic Degradation of Chromogenic Polymer Hydrogel Substrates
  • 06:02Conclusion

Summary

Tadução automática

A high-throughput assay for enzyme screening is described. This multiplexed ready-to-use assay kit comprises of pre-chosen Chromogenic Polymer Hydrogel (CPH) substrates and complex Insoluble Chromogenic Biomass (ICB) substrates. Target enzymes are polysaccharide degrading endo-enzymes and proteases.

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