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荧光活化细胞分选（FACS）和新鲜和冷冻大鼠脑组织的Fos表达神经元基因表达分析
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Neurociência
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JoVE Journal Neurociência
Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
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荧光活化细胞分选（FACS）和新鲜和冷冻大鼠脑组织的Fos表达神经元基因表达分析

DOI:
10.3791/54358-v

•

08:37 min

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August 27, 2016

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, , , ,
1Behavioral Neuroscience Research Branch, Intramural Research Program,NIDA, NIH, DHHS, 2Division of Rheumatology, School of Medicine,Johns Hopkins University

Capítulos

  • 00:05Título
  • 00:43Tissue Collection and Dissection
  • 02:17Cell Dissociation, Fixation and Permeabilization
  • 05:52Results: Representative Flow Cytometric and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Brain Tissue
  • 07:16Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们提出了一个荧光活化细胞分选（FACS）协议来研究分子改建的Fos表达来自新鲜和冷冻的脑组织神经元歌舞团。使用冷冻组织可在多个会话许多脑区的FACS隔离，最大限度地利用宝贵的动物主题。

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