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Der Nachweis von modifizierte Formen von Cytosin Mit Sensitive Immunhistochemie
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
DOI:

07:13 min

August 16, 2016

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Capítulos

  • 00:05Título
  • 00:47Fixation and Permeabilization of Cryo- and Microtome Sections
  • 01:34Immunostaining for Oxi-5mC Derivatives
  • 05:16Results: Immunohistochemical Analysis of 5hmC and 5caC Distributions in the Adult Mouse Brain Tissues
  • 06:12Conclusion

Summary

Tadução automática

Herein we describe a sensitive immunochemical method for mapping the spatial distribution of 5mC oxidation derivatives based on the use of peroxidase-conjugated secondary antibodies and tyramide signal amplification.

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