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נחישות כימות המינים בשנת תערובות שימוש ספקטרומטריית מסה MRM של פפטידים יישומית כדי אימות בשר
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
DOI:

09:26 min

September 20, 2016

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Capítulos

  • 00:05Título
  • 00:54Proteolysis and Analysis of Reference Myoglobins
  • 05:14Preparation and Analysis of Calibration Samples
  • 07:57Results: Example Outcomes for a Mixture of Horse in Beef
  • 08:33Conclusion

Summary

Tadução automática

We present a protocol for identifying and quantifying the components in mixtures of species possessing similar proteins. Mass spectrometry detects peptides for identification, and gives relative quantitation by ratios of peak areas. As a tool food for fraud detection, the method can detect 1% horse in beef.

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