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고기 인증에 펩티드 응용의 MRM 질량 분석을 사용하여 혼합물에서 종 결정 및 정량
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
DOI:

09:26 min

September 20, 2016

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Capítulos

  • 00:05Título
  • 00:54Proteolysis and Analysis of Reference Myoglobins
  • 05:14Preparation and Analysis of Calibration Samples
  • 07:57Results: Example Outcomes for a Mixture of Horse in Beef
  • 08:33Conclusion

Summary

Tadução automática

We present a protocol for identifying and quantifying the components in mixtures of species possessing similar proteins. Mass spectrometry detects peptides for identification, and gives relative quantitation by ratios of peak areas. As a tool food for fraud detection, the method can detect 1% horse in beef.

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