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T細胞への抗原提示細胞による抗原の提示を研究するための抗原駆動性大腸炎モデルの開発
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
DOI:

06:57 min

September 18, 2016

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Capítulos

  • 00:05Título
  • 01:28Colonic Lamina propria Cell Isolation
  • 03:58Results: Host CX3CR1+ Macrophages Communicate with Donor OT-II Cells During OVA-driven Transfer Colitis
  • 06:03Conclusion

Summary

Tadução automática

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

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