Journal
/
Biologia do Desenvolvimento
/
Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish

Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish

DOI:
10.3791/54431-v

•

09:40 min

•

October 31, 2016

•

, , ,
1School of Biological Sciences,Monash University

Capítulos

  • 00:05Título
  • 00:43Touch-evoked Response Assay
  • 01:53Quantification of Swimming Behavior
  • 03:3210 Min Swim Test Locomotion Assay
  • 07:01Results: Muscle Performance and Locomotion in Zebrafish Embryos
  • 08:47Conclusion

Summary

Tadução automática

Tadução automática

Zebrafish are an excellent model to study muscle function and disease. During early embryogenesis zebrafish begin regular muscle contractions producing rhythmic swimming behavior, which is altered when the muscle is disrupted. Here we describe a touch-evoked response and locomotion assay to examine swimming performance as a measure of muscle function.

Tags

Keywords: ZebrafishMuscle PerformanceTouch-evoked ResponseLocomotion AssayNeuromuscular ResearchHigh-throughputVideo RecordingMechanosensory StimulusSwimming BehaviorMaximum Acceleration

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/54431?article_type=v

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
check_url/pt/54431?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/pt/54431?article_type=v

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
check_url/pt/54431?article_type=v

Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules
check_url/pt/54431?article_type=v

Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations
check_url/pt/54431?article_type=v

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
check_url/pt/54431?article_type=v

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae
check_url/pt/54431?article_type=v

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
check_url/pt/54431?article_type=v

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development
check_url/pt/54431?article_type=v

High-Frequency Ultrasound Echocardiography to Assess Zebrafish Cardiac Function

Read Article