Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Robert J. Scarborough; Kelsey L. Adams; Olivier Del Corpo; A&#239;cha Daher; Anne Gatignol

doi:10.3791/54486

Journal

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Imunologia e Infecção

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Evaluering af effekt og toksicitet af RNA Målretning HIV-1 Produktion til brug i Gene eller Drug Therapy

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Evaluering af effekt og toksicitet af RNA Målretning HIV-1 Produktion til brug i Gene eller Drug Therapy

DOI:

10.3791/54486-v

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12:03 min

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September 05, 2016

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Robert J. Scarborough², Kelsey L. Adams², Olivier Del Corpo², Aïcha Daher, Anne Gatignol^2,3

¹Virus-Cell Interactions Laboratory,Lady Davis Institute for Medical Research, ²Department of Microbiology & Immunology,McGill University, ³Department of Medicine,McGill University

Capítulos

00:05Título
00:56Cells and Transfections
03:46Viral Production Assay
05:38Cell Viability Assay
06:28Immune Activation Assay
08:59Results: Evaluation of siRNA Targeting HIV-1 RNA
10:34Conclusion

Summary

Tadução automática

Methods to evaluate the efficacy and toxicity of RNA molecules targeting post-integration steps of the HIV-1 replication cycle are described. These methods are useful for screening new molecules and optimizing the format of existing ones.

Evaluering af effekt og toksicitet af RNA Målretning HIV-1 Produktion til brug i Gene eller Drug Therapy

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