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Biologia do Desenvolvimento
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Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

DOI:
10.3791/54492-v

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11:19 min

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December 16, 2016

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, , ,
1Laboratory of Genetics,University of Wisconsin, 2Department of Neurobiology,University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program,University of Pittsburgh School of Medicine

Capítulos

  • 00:05Título
  • 00:47Selecting Females for Egg Collection via Interrupted Mating
  • 01:31Preparation of Sperm Solution
  • 02:50UV Treatment of Sperm Solution
  • 03:37Manual Extrusion of Mature Eggs
  • 04:56In Vitro Fertilization
  • 05:51Heat Shock Treatment
  • 07:13Selection of Embryos with a One-cycle Cytokinesis Stall
  • 08:48Results: Heat Shock 2 Treatment of Zebrafish Embryos
  • 09:57Conclusion

Summary

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Tadução automática

A modified protocol for ploidy manipulation uses a heat shock to induce a one-cycle stall in cytokinesis in the early embryo. This protocol is demonstrated in the zebrafish but may be applicable to other species.

Tags

Keywords: Ploidy ManipulationZebrafish EmbryosHeat ShockDiploidizationGenéticaInbred LinesGenetic ManipulationsMatingEgg ExtrusionSperm SolutionUV TreatmentDissectionTestesHank SolutionPipettingUV Lamp

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