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自旋饱和转移差异核磁共振（NMR SSTD）：一种新的工具来获得化学交换过程的动力学参数
JoVE Journal
Química
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JoVE Journal Química
Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
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自旋饱和转移差异核磁共振（NMR SSTD）：一种新的工具来获得化学交换过程的动力学参数

DOI:
10.3791/54499-v

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11:44 min

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November 12, 2016

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, , ,
1School of Chemistry,University of East Anglia, 2School of Pharmacy,University of East Anglia

Capítulos

  • 00:05Título
  • 01:09Nuclear Magnetic Resonance (NMR) Sample Preparation and Experimental Setup
  • 03:50SSTD NMR Experiment Acquisition
  • 06:42SSTD NMR Experiment Processing
  • 08:53Results: Calculation of Kinetic Parameters in the Rotation of the Amide Bond of N,N-dimethylacetamide via the SSTD NMR Technique
  • 10:28Conclusion

Summary

Tadução automática

Tadução automática

A detailed protocol describing the SSTD NMR method is presented here to help new users apply this new method to obtain the kinetic parameters of their own systems undergoing chemical exchange.

Tags

Keywords: Spin Saturation Transfer Difference NMR (SSTD NMR)Chemical ExchangeKinetic ParametersMolecule DynamicsOrganic MoleculesOrganometallic MoleculesRotational VarianceConformational EquilibriumNitrogen InversionLignin ExchangeTautomerizationTemperature ControlProton NMRSTDDIFF Pulse Sequence90 Degree Hard Pulse

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