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通过注射心脏毒素急性骨骼肌再生的诱导
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
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通过注射心脏毒素急性骨骼肌再生的诱导

DOI:
10.3791/54515-v

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07:39 min

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January 01, 2017

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, , , , ,
1Stem Cell Fate Laboratory,Institute of Genetics and Biophysics “A. Buzzati Traverso”, CNR, 2School of Medicine and Surgery,University of Milano-Bicocca, 3Division of Immunology, Transplantation and Infectious Diseases,San Raffaele Scientific Institute

Capítulos

  • 00:05Título
  • 00:35Cardiotoxin Injection in the Tibialis Anterior Muscle
  • 01:29Tibialis Anterior Isolation
  • 02:51Fresh Frozen Muscle Technique
  • 04:03Cryostat Sectioning of Frozen Muscles
  • 04:46Hematoxylin & Eosin Stains
  • 05:57Results: Time Course Analysis of Skeletal Muscle Regeneration
  • 07:04Conclusion

Summary

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Tadução automática

该原稿描述了详细的协议来诱导成年小鼠和肌肉的随后的操作，如解剖，冷冻，切割，常规染色，和肌纤维横截面积分析急性骨骼肌再生。

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