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Biologia do Desenvolvimento
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Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

DOI:
10.3791/54515-v

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07:39 min

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January 01, 2017

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, , , , ,
1Stem Cell Fate Laboratory,Institute of Genetics and Biophysics “A. Buzzati Traverso”, CNR, 2School of Medicine and Surgery,University of Milano-Bicocca, 3Division of Immunology, Transplantation and Infectious Diseases,San Raffaele Scientific Institute

Capítulos

  • 00:05Título
  • 00:35Cardiotoxin Injection in the Tibialis Anterior Muscle
  • 01:29Tibialis Anterior Isolation
  • 02:51Fresh Frozen Muscle Technique
  • 04:03Cryostat Sectioning of Frozen Muscles
  • 04:46Hematoxylin & Eosin Stains
  • 05:57Results: Time Course Analysis of Skeletal Muscle Regeneration
  • 07:04Conclusion

Summary

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This manuscript describes a detailed protocol to induce acute skeletal muscle regeneration in adult mice and subsequent manipulations of the muscles, such as dissection, freezing, cutting, routine staining, and myofiber cross-sectional area analysis.

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