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斜脊髓切片用于神经前根刺激的制备

JoVE Journal
Neurociência

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JoVE Journal Neurociência

The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation

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斜脊髓切片用于神经前根刺激的制备

DOI:

10.3791/54525-v

•

09:10 min

•

October 13, 2016

•

Félix Leroy, Boris Lamotte d’Incamps

¹Centre National de la Recherche Scientifique (UMR 8119),Centre de Neurophysique, Physiologie et Pathologie, Université Paris Descartes

Capítulos

00:05Título
00:43Spinal Cord Dissection
02:41Agar Embedding
03:50Sectioning the Spinal Cord
05:02Preparation for Patch-Clamp Recording
06:40Results: Motoneuron Response to Ventral Root Stimulation
08:28Conclusion

Summary

Tadução automática

我们显示如何在年轻小鼠制备脊髓倾斜切片。该制剂允许前根的刺激。

Tags

Keywords: Spinal Cord Slices Ventral Root Stimulation Motoneuron Identification Dissection Laminectomy Dura Removal Orientation Marking Agar Embedding

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