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的pH依赖性活性的检测<em>大肠杆菌</em>陪护HdeB<em>体外</em>和<em>在体内</em

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

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的pH依赖性活性的检测<em>大肠杆菌</em>陪护HdeB<em>体外</em>和<em>在体内</em

DOI:

10.3791/54527-v

•

08:32 min

•

October 23, 2016

•

Jan-Ulrik Dahl, Philipp Koldewey², James C. A. Bardwell², Ursula Jakob

¹Department of Molecular, Cellular, and Developmental Biology,University of Michigan, ²Howard Hughes Medical Institute,University of Michigan

Capítulos

00:05Título
01:16Chaperone Activity Assay Using Thermally Unfolding Malate Dehydrogenase (MDH)
03:18Effect of HdeB Overexpression on E. coli Survival under Acid Stress
05:14Results: Chaperone Activity Assay Using Thermally Unfolded MDH and the Effect of HdeB Overexpression on E. coli Survival under Acid Stress
07:16Conclusion

Summary

Tadução automática

这项研究说明生物物理，生物化学和分子生物学技术的酸性pH条件下表征大肠杆菌 HdeB的伴侣活性。这些方法已被成功地用于其它酸保护伴侣分子如HdeA蛋白，并且可以进行修改，以用于其它分子伴侣和应力的条件下工作。

Tags

Escherichia Coli Chaperone HdeB PH-dependent Activity In Vitro In Vivo Acid Activated Chaperones Protein Unfolding Protein Degradation Enteric Bacteria Stomach Acid Protective Chaperones HDA Malate Dehydrogenase Light Scattering Fluorescent Spectrophotometer Temperature Control

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