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Fremgangsmåde til at identificere småmolekylære inhibitorer af protein-protein-vekselvirkning mellem HCN1 og TRIP8b
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Bioquímica
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JoVE Journal Bioquímica
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Fremgangsmåde til at identificere småmolekylære inhibitorer af protein-protein-vekselvirkning mellem HCN1 og TRIP8b

DOI:
10.3791/54540-v

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10:20 min

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November 11, 2016

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, , , , , , ,
1Davee Department of Neurology and Clinical Neurosciences,Feinberg School of Medicine, Northwestern University, 2Center for Molecular Innovation and Drug Discovery,Northwestern University, 3Department of Pharmacology,Feinberg School of Medicine, Northwestern University, 4High Throughput Analysis Laboratory, Department of Molecular Biosciences,Northwestern University, 5Department of Physiology,Feinberg School of Medicine, Northwestern University

Capítulos

  • 00:05Título
  • 01:08High Throughput Screen
  • 03:07Confirmation of Hits Using TAMRA-tagged HCN1 Peptide
  • 04:33Dose-response Validation of Bead-based Proximity Assay
  • 07:15Results: Identification of Small Molecule Inhibitors Between HCN1 and TRIPb8 Interactions
  • 09:14Conclusion

Summary

Tadução automática

Tadução automática

Samspillet mellem HCN kanaler og deres ekstra subunit er blevet identificeret som en terapeutisk mål i depression. Her, en fluorescenspolarisering-baseret fremgangsmåde til identifikation af småmolekylære inhibitorer af dette protein-protein-interaktion, er præsenteret.

Tags

Keywords: Small Molecule InhibitorsProtein-protein InteractionHCN1TRIP8bHigh-throughput ScreeningFluorescence PolarizationInhibition AssayDepressionTherapeutic TargetAcoustic Liquid Handler384-well PlateFITC-labeled HCN1TAMRA-labeled HCN1

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