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Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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Capítulos
- 00:05Título
- 00:41High Capacity Magnetic Protein A or G Bead Antibody Capture
- 01:43Antibody Labeling Using Amine Chemistry
- 02:56Antibody Labeling Using Thiol Chemistry
- 04:46Results: Magnetic BEad Fluorescent Dye Labeling Results in a Highly Efficient Purified Antibody Recovery
- 05:39Conclusion
The on-bead method for labeling antibodies with small molecules enables labeling of a small amount of antibodies directly from cell media. This method is compatible with amine and thiol chemistry, and can handle multiple samples in parallel, manually or using automated platforms.
