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זיהום הניסיון עם<em> חיידקי ליסטריה</em> כמודל תגובות לימוד מארח אינטרפרון-γ
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Capítulos
- 00:05Título
- 00:54Glycerol Stock Long-term Storage Preparation
- 02:18Experimental L. monocytogenes Inoculum Preparation and Infection
- 03:44Peak Infection Spleen and Liver Bacterial Load Measurement
- 06:16Effects of L. monocytogenes on CD4+ and CD8+ Cell IFN-gamma Responses
- 07:43Results: Representative NK, NKT, and CD4 and CD8 T cell IFN-gamma Responses to L. monocytogenes
- 08:54Conclusion
This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.
