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Produktionen af ​​pluripotente stamceller fra mus fostervand Celler Ved hjælp af en Transposon System
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Produktionen af ​​pluripotente stamceller fra mus fostervand Celler Ved hjælp af en Transposon System

DOI:
10.3791/54598-v

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08:24 min

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February 28, 2017

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, , , , , ,
1Stem Cell and Regenerative Medicine Laboratory,Fondazione Istituto di Ricerca Pediatrica Citta della Speranza, 2Lunenfeld-Tanenbaum Research Institute,Mount Sinai Hospital, 3Stem Cells and Regenerative Medicine Section, Developmental Biology and Cancer Programme,UCL Institute of Child Health and Great Ormond Street Hospital

Capítulos

  • 00:05Título
  • 00:58Mouse Amniotic Fluid (AF) Cell Isolation
  • 03:24Transfection of Mouse AF Cells
  • 04:24Induced Pluripotent Stem (iPS)-AF Cell Genreation and Culture
  • 06:32Results: Reprogramming of Mouse Amniotic Fluid Cells
  • 07:50Conclusion

Summary

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In this study, we generate induced pluripotent stem cells from mouse amniotic fluid cells, using a non-viral-based transposon system.

Tags

Mouse Amniotic Fluid CellsPluripotent Stem CellsTransposon SystemReprogrammingFetal TherapyCell IsolationCell CultureMEF Feeder Cells

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