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急性视网膜模型评估血视网膜屏障违约和潜在的药物治疗
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JoVE Journal Medicina
An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment
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急性视网膜模型评估血视网膜屏障违约和潜在的药物治疗

DOI:
10.3791/54619-v

•

09:33 min

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September 13, 2016

•

, , ,
1Graduate School of Biomedical Sciences,Rowan University, 2Department of Cell Biology,Rowan University School of Osteopathic Medicine

Capítulos

  • 00:05Título
  • 00:52Retinal Organotypic Culture
  • 02:31Preparing Samples for Immunostaining
  • 04:03Immunostaining
  • 05:01Image Analysis
  • 06:41Results: The Blood Retinal Barrier is Compromised upon Histamine Exposure and Rescued by LXA4 Treatment
  • 08:22Conclusion

Summary

Tadução automática

Tadução automática

一个低成本，易于使用和强大的制度的建立，以评估可能改善组胺引起血视网膜屏障破坏的潜在治疗。血管渗漏，穆勒细胞活化和神经过程的连续性被用来评估损伤反应及其逆转与潜在的药物，脂氧素A4。

Tags

Blood Retinal BarrierNeural Degenerative DiseasesEx Vivo Retinal ModelDrug ScreeningRetina DissectionHistamineLXA4PFASucroseFreezing Medium

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