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De Algemene Vergadering en de toepassing van 'Shear Rings': A Novel endotheliale Model voor Orbital, Unidirectionele en Periodieke Fluid Flow en Shear Stress
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Biologia
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JoVE Journal Biologia
The Assembly and Application of ‘Shear Rings’: A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
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De Algemene Vergadering en de toepassing van 'Shear Rings': A Novel endotheliale Model voor Orbital, Unidirectionele en Periodieke Fluid Flow en Shear Stress

DOI:
10.3791/54632-v

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09:20 min

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October 31, 2016

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, , , , , , , ,
1Molecular and Cellular Physiology,Louisiana State University Health Sciences Center in Shreveport, 2Biological Sciences,Louisiana Tech University, 3Neurology,Louisiana State University Health Sciences Center in Shreveport, 4Institut Cochin, Inserm U1016, Cnrs Umr8104,Université Paris Descartes

Capítulos

  • 00:05Título
  • 00:45Construction of 150-mm Diameter Shear Rings
  • 04:57Sterilization of Shear Rings
  • 06:31Shear Stress Studies
  • 07:40Results: Shear Rings Effectively Create Unidirectional and Periodic Shear Stress
  • 08:29Conclusion

Summary

Tadução automática

Tadução automática

Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.

Tags

Shear RingsEndothelial ModelFluid FlowShear StressMethylene ChloridePetri DishAdhesionPeriodic Flow

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