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Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres
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Capítulos
- 00:05Título
- 00:59Compound of Interest (COI)-loaded Microsphere Preparation
- 03:01COI-loaded Islet Treatment and Intact Islet Dispersion
- 05:40Dissociated Islet Immunofluorescent Labeling
- 07:07Results: Representative Quantification and Analysis of β-cell Proliferation
- 08:11Conclusion
Here, we present methodology to generate and administer compound of interest-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres to intact mouse islets in culture with subsequent immunofluorescence analysis of β-cell proliferation. This method is suitable for determining the efficacy of candidate β-cell mitogens.
