É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 00:50Transfection of TFO-directed ICL-containing Plasmids in Human Cells
- 02:27Crosslinking and DNA Isolation
- 05:21Chromatin Immunoprecipitation of TFO-directed ICL-containing Plasmids
- 07:06DNA Supercoiling Assay and Two Dimensional Agarose Gel Electrophoresis
- 09:30Studying DNA Topology Modification Using ChIP and DNA Supercoiling Assays
- 10:45Conclusion
Targeted DNA damage can be achieved by tethering a DNA damaging agent to a triplex-forming oligonucleotide (TFO). Using modified TFOs, DNA damage-specific protein association, and DNA topology modification can be studied in human cells by the utilization of modified chromatin immunoprecipitation assays and DNA supercoiling assays described herein.
