Journal
/
/
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
DOI:

08:48 min

October 25, 2016

, , , , , , , , ,

Capítulos

  • 00:05Título
  • 00:35T Cell Receptor (TCR) Hemichain-encoded Retroviral Construct Preparation
  • 01:41Stable Packaging Cell Line Generation
  • 03:08Human T Cell Activation and Transduction
  • 05:04Novel Antigen-specific TCR Clone Reconstitution
  • 06:25Results: Centric TCR Hemichains Pair with Different Counter-chains to Form Antigen-specific TCRs with Varying Affinity
  • 07:59Conclusion

Summary

Tadução automática

Herein we describe a novel method to generate antigen-specific T cell receptors (TCRs) by pairing the TCRα or TCRβ of an existing TCR, possessing the antigen-specificity of interest, with complementary hemichain of the peripheral T cell receptor repertoire. The de novo generated TCRs retain antigen-specificity with varying affinity.

Vídeos Relacionados

Read Article