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发电<em>德诺</em>抗原特异性人T细胞受体通过中心的Hemichain的逆转录病毒转导

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

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发电<em>德诺</em>抗原特异性人T细胞受体通过中心的Hemichain的逆转录病毒转导

DOI:

10.3791/54697-v

•

08:48 min

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October 25, 2016

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Tingxi Guo², Toshiki Ochi*², Munehide Nakatsugawa*², Yuki Kagoya, Mark Anczurowski², Chung-Hsi Wang², Muhammed A. Rahman, Kayoko Saso, Marcus O. Butler², Naoto Hirano²

¹Department of Immunology,University of Toronto, ²Princess Margaret Cancer Centre,University Health Network

Capítulos

00:05Título
00:35T Cell Receptor (TCR) Hemichain-encoded Retroviral Construct Preparation
01:41Stable Packaging Cell Line Generation
03:08Human T Cell Activation and Transduction
05:04Novel Antigen-specific TCR Clone Reconstitution
06:25Results: Centric TCR Hemichains Pair with Different Counter-chains to Form Antigen-specific TCRs with Varying Affinity
07:59Conclusion

Summary

Tadução automática

这里我们描述了一种新方法，以产生抗原特异性T细胞受体（TCR）通过配对现有的TCR的TCRα或TCRβ，具有感兴趣的抗原特异性，与外周T细胞受体库的补充hemichain。从头产生的TCR保留抗原特异性有不同的亲和力。

Tags

Keywords: De Novo T Cell Receptor TCR Hemi-chain Retroviral Transduction Antigen-specific T Cells Gene Engineering Immunotherapy PCR Cloning Bacterial Transformation Plasmid Purification 293GPG Cell Transfection PG-13 Packaging Cell Line PBMC Activation

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