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L'étude de la protéine système de contrôle de la qualité des<em> D. discoideum</em> Utilisation de cellules vivantes d'imagerie à température contrôlée
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging
DOI:

06:09 min

December 02, 2016

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Capítulos

  • 00:05Título
  • 00:35Cell Preparation
  • 01:19Imaging
  • 02:53Image Analysis
  • 04:05Results: Behavior of Aggregation-prone Prion-like Proteins in D. discoideum during Heat Stress
  • 05:25Conclusion

Summary

Tadução automática

The social amoebae Dictyostelium discoideum has recently been established as a system to study protein misfolding and proteostasis. Here, we describe a new imaging-based methodology to study temperature-induced protein aggregation and the cellular stress response in D. discoideum.

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