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Capítulos
- 00:05Título
- 00:43Collect Eggs from Flies with Genotype of Interest
- 02:11Transfer Larvae to Experimental Food
- 03:05Determine Larvae Fat Levels
- 06:15Results: Neuronal Function in Specific Regions of the Larval Brain Is Necessary and Sufficient for Body Fat Regulation
- 07:01Conclusion
Here we present a method to measure organismal fat levels in the third instar (L3) larval stage of Drosophila melanogaster. This method exploits the comparatively low density of fat tissue to differentiate between larvae with altered fat stores. Buoyancy-based analysis is a valuable tool for rapid, reproducible, and economical screening.
