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JoVE Journal Biochemistry
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
 

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Article DOI: 10.3791/54795-v • 09:02 min • November 10th, 2016
November 10th, 2016 •
*1, *1, 1, 1
1Biological Engineering Department, Utah State University
* These authors contributed equally

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Nanoparticle-based optical probes have been designed as a vehicle for detecting antigens using Raman and UV-Vis spectroscopy. Here we describe a protocol for preparing such probes for a UV-Vis/Raman spectroscopy immunoassay in such a way to incorporate future multiplexing capabilities.

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UV-Vis Raman Spectroscopy Immunoassay Platform Gold Nano Particles Optical Methods Assay Validation Functionalization Optical Absorption Light Scattering Customizable Platform Biomarkers Bi-functional Crosslinking Agent Pyridyl Disulfide PEG-NHS Sodium Bicarbonate Antibody Solution
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