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从HIV-1感染细胞的外来体基于SILAC蛋白质组学表征

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

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从HIV-1感染细胞的外来体基于SILAC蛋白质组学表征

DOI:

10.3791/54799-v

•

10:24 min

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March 03, 2017

•

Collins Cheruiyot, Zemplen Pataki, Robert Williams, Bharat Ramratnam³, Ming Li

¹Brown University, ²COBRE Center for Cancer Research, Lifespan Laboratories,Rhode Island and Miriam Hospitals, ³Division of Infectious Diseases, Department of Medicine,Warren Alpert Medical School, Brown University

Capítulos

00:05Título
00:41Cell Culture and HIV-1 Infection
02:11Exosome Isolation
03:42Protein Extraction
04:58Proteomic Data Analysis
05:43Bioinformatics Verification and Characterization
08:20Results: SILAC Is an Effective Method for Analyzing the Effects of HIV-1 Infection on Host Exosomal Proteomes
09:08Conclusion

Summary

Tadução automática

在这里，我们描述了使用通过在细胞培养物（SILAC）氨基酸稳定同位素标记的技术来分析HIV-1感染的主机外来体蛋白质组的作用的定量蛋白质组学方法。该协议可以很容易地适应于不同的压力或感染的条件下的细胞。

Tags

Keywords: SILAC Proteomic Characterization Exosomes HIV-1 Infection Cell Culture Labeling Ultracentrifugation Mass Spectrometry

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