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大众组织学量化在神经退行性变<em>果蝇</em
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Neurociência
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JoVE Journal Neurociência
Mass Histology to Quantify Neurodegeneration in Drosophila
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大众组织学量化在神经退行性变<em>果蝇</em

DOI:
10.3791/54809-v

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06:34 min

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December 15, 2016

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,
1Oregon Institute of Occupational Health Sciences,Oregon Health & Sciences University

Capítulos

  • 00:05Título
  • 00:58Fixing and Paraffin-embedding Drosophilia Heads
  • 02:36Sectioning, Mounting, and Microscopy
  • 04:38Results: Mass Histology Allows for Large-scale Analysis of Neurodegeneation in Drosophila
  • 05:59Conclusion

Summary

Tadução automática

Tadução automática

果蝇被广泛用作模型系统来研究神经变性。这个协议描述由退化，如由大脑液泡形成确定的，可量化的方法。它也最大限度地减少的效果因的实验程序由处理和切片对照和实验苍蝇，因为一个样本。

Tags

Keywords: Mass HistologyNeurodegenerationDrosophilaBrainNeurodegenerative DiseasesGenetic ModificationsEnvironmental InfluencesStainingForcepsCollarsEyeless Senoculus FliesCarnoy’s SolutionEthanolMethyl BenzoateParaffinEmbeddingParaffin BlocksSectioningHeating PlateRazor BladesMounting Blocks

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