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可视化Interrenal类固醇合成组织及其血管微环境斑马鱼
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
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可视化Interrenal类固醇合成组织及其血管微环境斑马鱼

DOI:
10.3791/54820-v

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07:19 min

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December 21, 2016

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, , ,
1Department of Life Science,Tunghai University

Capítulos

  • 00:05Título
  • 01:04Whole Mount 3β-Hsd Activity Staining
  • 03:15IHC Analysis of 3β-Hsd Activity-stained Embryos
  • 05:28Results: Zebrafish Interrenal Steroidogenic Tissue
  • 06:30Conclusion

Summary

Tadução automática

Tadução automática

在斑马鱼的interrenal腺是哺乳动物的肾上腺的硬骨鱼类的对应。此协议引入了如何执行3-β羟基类固醇脱氢酶（Δ5-4异构酶;3β-HSD）的酶活性测定法，其检测在显影斑马鱼分化类固醇合成的细胞。

Tags

Vascular MicroenvironmentZebrafishEnzymatic Activity AssayWhole-mount StainingTransgenic Fluorescent Reporter LinesVibratome SectioningImmunohistochemistryConfocal Microscopy3beta-Hsd Activity StainingPigment InhibitionPFA FixationPBST WashingChromogenic Signal DetectionPrecipitationPost-fixationGlycerol ClearingWhole-mount Microscopy

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