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De alto rendimiento, en tiempo real, Prueba de doble lectura de Intracelular Actividad antimicrobiana y la citotoxicidad celular eucariótica
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Capítulos
- 00:05Título
- 01:30Real Time Intracellular Growth and Eukaryotic Cell Cytotoxicity Assay
- 03:14Preparing Luminescent or Fluorescent Legionella pneumophila and Macrophage Infection
- 04:49Assay Readout, and Single and Multi-dimensional Dose-response Testing and Data Interpretation
- 06:09Results: Simultaneous Real-time Assay Results for Intracellular Bacterial Growth and Eukaryotic Cell Cytotoxicity
- 07:41Conclusion
A high throughput, real-time assay was developed to simultaneously identify (1) eukaryotic cell-penetrant antimicrobials targeting an intracellular bacterial pathogen, and (2) assess eukaryotic cell cytotoxicity. A variation on the same technology was thereafter combined with digital dispensing technology to enable facile, high-resolution, dose-response, and two- and three-dimensional synergy studies.
