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Udvikling af en hepatitis B virus Reporter system til at overvåge tidlige stadier af Replication Cycle
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Udvikling af en hepatitis B virus Reporter system til at overvåge tidlige stadier af Replication Cycle

DOI:
10.3791/54849-v

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09:35 min

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February 01, 2017

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, , , , ,
1Research Center for Hepatitis and Immunology,National Center for Global Health and Medicine, 2Central Pharmaceutical Research Institute,Japan Tobacco Inc.

Capítulos

  • 00:05Título
  • 00:42Production of Recombinant Hepatitis B Virus Encoding the Reporter Protein
  • 03:33Infection of Recombinant Hepatitis B Virus
  • 05:09Analysis of Hepatitis B Virus Infection
  • 05:56Results: HBV Reporter Activity in HepG2 Cells Infected with Recombinant Hepatitis B Virus
  • 07:57Conclusion

Summary

Tadução automática

Tadução automática

Her beskriver vi en nyudviklet hepatitis B virus (HBV) reporter-system til at overvåge de tidlige stadier af HBV livscyklus. Denne forenklede in vitro-system vil støtte i screening af anti-HBV-midler ved anvendelse af en high-throughput strategi.

Tags

Keywords: Hepatitis B VirusHBVReporter SystemHepG2 CellsTransfectionRecombinant HBVCell CultureVirus ReplicationVirus PurificationNTCPInfection

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