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Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
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Biologia
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JoVE Journal Biologia
Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

DOI:
10.3791/54874-v

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13:35 min

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May 05, 2017

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,
1Institut de Biochimie et Génétique Cellulaires, Centre National de la Recherche Scientifique, UMR 5095,Université de Bordeaux

Capítulos

  • 00:00Título
  • 01:38Preparation of Formvar Grid Film
  • 03:53Preparation of Freeze-substitution Medium
  • 05:06Preparation of Cells
  • 06:11Liquefaction of Propane
  • 07:32Plunge Freezing of Samples
  • 08:32Freeze-substitution
  • 09:04Sample Warming
  • 10:57Results: Plunge Freezing Results in Improved Preservation of the Intracellular Structures
  • 12:50Conclusion

Summary

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This manuscript describes an easy-to-use and low-cost cryofixation method for visualizing suspension cells by transmission electron microscopy.

Tags

Plunge FreezingUltrastructural StudiesImmunolocalizationSuspension CellsTransmission Electron MicroscopyFreeze SubstitutionChemical FixationCryofixationFormvar FilmGlass Slide

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